4V310-10-NC代理AIRTAC電磁閥4V310-10-NC

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代理AIRTAC電磁閥4V310-10-NC 4V210-08-NC 現(xiàn)貨銷售

產品參數(shù)：

品牌：AIRTAC

型號：4V310-10

電壓：AC220V

材質：鋁

接管口徑：10MM

電磁閥工作原理：

AIRTAC電磁閥從原理上分為三大類：

（1）直動式電磁閥：原理：通電時，電磁線圈產生電磁力把關閉件從閥座上提起，閥門打開；斷電時，電磁力消失，彈簧把關閉件壓在閥座上，閥門關閉。特點：在真空、負壓、零壓時能正常工作，但通徑一般不超過25mm。

（2）分步直動式電磁閥：原理：它是一種直動和先導式相結合的原理，當入口與出口沒有壓差時，通電后，電磁力直接把先導小閥和主閥關閉件依次向上提起，閥門打開。當入口與出口達到啟動壓差時，通電后，電磁力先導小閥，主閥下腔壓力上升，上腔壓力下降，從而利用壓差把主閥向上推開；斷電時，先導閥利用彈簧力或介質壓力推動關閉件，向下移動，使閥門關閉。特點：在零壓差或真空、高壓時亦能可*動作，但功率較大，要求必須水平安裝。

（3）先導式電磁閥：原理：通電時，電磁力把先導孔打開，上腔室壓力迅速下降，在關閉件周圍形成上低下高的壓差，流體壓力推動關閉件向上移動，閥門打開；斷電時，彈簧力把先導孔關閉，入口壓力通過旁通孔迅速腔室在關閥件周圍形成下低上高的壓差，流體壓力推動關閉件向下移動，關閉閥門。特點：流體壓力范圍上限較高，可任意安裝（需定制）但必須滿足流體壓差條件。2.電磁閥從閥結構和材料上的不同與原理上的區(qū)別，分為六個分支小類：直動膜片結構、分步直動膜片結構、先導膜片結構、直動活塞結構、分步直動活塞結構、先導活塞結構。

 AIRTAC電磁閥的主要特點：   （1）外漏堵絕，內漏易控，使用安全。內外泄漏是危及安全的要素。其它自控閥通常將閥桿伸出，由電動、氣動、液動執(zhí)行機構控制閥芯的轉動或移動。這都要解決長期動作閥桿動密封的外泄漏難題；唯有電磁閥是用電磁力作用于密封在電動調節(jié)閥隔磁套管內的鐵芯完成，不存在動密封，所以外漏易堵絕。電動閥力矩控制不易，容易產生內漏，甚至拉斷閥桿頭部；電磁閥的結構型式容易控制內泄漏，直至降為零。所以，電磁閥使用特別安全，尤其適用于腐蝕性、有毒或高低溫的介質。  （2）系統(tǒng)簡單，便接電腦，價格低謙。電磁閥本身結構簡單，價格也低，比起調節(jié)閥等其它種類執(zhí)行器易于安裝維護。更顯著的是所組成的自控系統(tǒng)簡單得多，價格要低得多。由于電磁閥是開關信號控制，與工控計算機連接十分方便。在當今電腦普及，價格大幅下降的時代，電磁閥的優(yōu)勢就更加明顯。

  （3）動作快遞，功率微小，外形輕巧。電磁閥響應時間可以短至幾個毫秒，即使是先導式電磁閥也可以控制在幾十毫秒內。由于自成回路，比之其它自控閥反應更靈敏。設計得當?shù)碾姶砰y線圈功率消耗很低，屬節(jié)能產品；還可做到只需觸發(fā)動作，自動保持閥位，平時一點也不耗電。電磁閥外形尺寸小，既節(jié)省空間，又輕巧美觀。  （4）調節(jié)精度受限，適用介質受限。電磁閥通常只有開關兩種狀態(tài)，閥芯只能處于兩個極限位置，不能連續(xù)調節(jié)，（力圖突破的新構思不少，但還都處于試驗試用階段）所以調節(jié)精度還受到一定限制。電磁閥對介質潔凈度有較高要求，含顆粒狀的介質不能適用，如屬雜質須先濾去。另外，粘稠狀介質不能適用，而且，特定的產品適用的介質粘度范圍相對較窄。

  （5）型號多樣，用途廣泛。電磁閥雖有先天不足，優(yōu)點仍十分突出，所以就設 1[1]計成多種多樣的產品，滿足各種不同的需求，用途極為廣泛。電磁閥技術的進步也都是圍繞著如何克服先天不足，如何更好地發(fā)揮固有優(yōu)勢而展開。AIRTAC電磁閥是用來控制流體方向的自動化基礎元件，屬于執(zhí)行器；通常用于機械控制和工業(yè)閥門上面，對介質方向進行控制，從而達到對閥門開關的控制。工作原理電磁閥里有密閉的腔，在不同位置開有通孔，每個孔都通向不同的油管，腔中間是閥，兩面是兩塊電磁鐵，哪面的磁鐵線圈通電閥體就會被吸引到哪邊，通過控制閥體的移動來擋住或漏出不同的排油的孔，而進油孔是常開的，液壓油就會進入不同的排油管，然后通過油的壓力來推動油缸的活塞，活塞又帶動活塞桿，活塞桿帶動機械裝置動。這樣通過控制電磁鐵的電流通斷就控制了機械運動。

代理銷售AIRTAC電磁閥4V310-10 4V230C-08 4V310-10-NC 4V210-08-NC

 

HGH15CA，HGH15CAHIWIN直線導軌，HGH15CA滑塊,HGH15CA導軌

東莞市至揚五金配件有限公司是一家經銷臺灣、日本、韓國、德國直線傳動部件的貿易商，代理經銷的產品有：直線導軌，滾珠絲桿,精密模組，支撐座配件，交叉導軌等等，同時兼營銷售國內外名優(yōu)軸承，國產五金配件等。公司以來秉承“客戶至上，服務到位”的原則在珠三角乃至全國業(yè)內廣受好評，今后必將堅持這一理念，持續(xù)經營，薄利多銷回饋廣大消費者及同行。主要品牌有：直線導軌：HIWIN  CPC  PMI  STAF  LSK  THK  IKO  力士樂  INA  SBC及國產名優(yōu)導軌等等。滾珠絲桿：HIWIN  PMI   TBI   THK   GTEN   合慶 及國產名優(yōu)絲桿。交叉導軌：THK  IKO  GMT  MID  及國內名優(yōu)交叉導軌產品。特種軸承：塑料軸承  不銹鋼軸承  陶瓷軸承  混合軸承  微型軸承。滑臺系列：GMT  THK  IKO  MID.以上為常用品牌及產品，其他未注明品牌，型號歡迎來電咨詢 HIWIN直線導軌滑塊系列型號HGH15CA HGH20CA HGH25CA HGH30CA HGH35CA HGH45CA HGH55CA HGH65CAHGW15CA HGW20CA HGW25CA HGW30CA HGW35CA HGW45CA HGW55CA HGW65CAHGW15CC HGW20CC HGW25CC HGW30CC HGW35CC HGW45CC HGW55CC HGW65CCHGH20HA HGH25HA HGH30HA HGH35HA HGH45HA HGH55HA HGH65HAHGW20HC HGW25HC HGW30HC HGW35HC HGW45HC HGW55HC HGW65HCHGW20HA HGW25HA HGW30HA HGW35HA HGW45HA HGW55HA HGW65HAEGH15CA EGH20CA EGH25CA EGH30CA EGH15SA EGH20SA EGH25SA EGH30SAEGW15CA EGW20CA EGW25CA EGW30CA EGW15SA EGW20SA EGW25SA EGW30SA

HIWIN微型導軌滑塊型號MGN7C MGN9C MGN12C MGN15C MGN7H MGN9H MGN12H MGN15HMGW7C MGW9C MGW12C MGW15C MGW7H MGW9H MGW12H MGW15H 

該公司產品分類： DA-AA/AAL/BA/BAL系列 DA-C/CL系列 HJG漢江滑塊，導軌 LGS系列導軌，滑塊 LG系列導軌，滑塊 HTPM滑塊，導軌 博特直線導軌 TBI直線導軌 CPC直線導軌 ABBA直線導軌 HIWIN模組 HIWIN直線導軌 THk直線導軌

HR-250AZAND精密電子天平廠【252g電子天平 HR-250AZ】

 

AND精密電子天平廠【252g電子天平 HR-250AZ】

產征：

快速２穩(wěn)定

可拆裝大型防靜電弧形防風罩

精巧外型設計：198 mm × 294 mm

旋轉滑動門設計，寬敞的稱量空間

內置校準砝碼，自動自校準（ＨＲ－ＡＺ）

高清晰背光源液晶顯示

１４種稱重單位，內含用戶自編程的稱量單位

ＧＬＰ/ＧＭＰ/ＧＣＰ/ＩＳＯ格式

ＡＣＡＩ自動計數(shù)精度提高功能

百分比功能

自動環(huán)境調節(jié)功能＆保持功能

統(tǒng)計計數(shù)功能

帶蜂鳴器比較輸出

自動開關機功能

下掛鉤功能

密度測量功能

 

鼎拓衡器以滿足用戶的需要為發(fā)展方向，為不僅廣大用戶提供更多功能強大，性能格比優(yōu)越產，還將致力于為廣大用戶提供售咨詢、售中輔導、售后培訓等服務。方便快捷的滿足需求，及時有效的解決疑惑是我們不變的經營宗旨。

 

備注：網上報均為參考，產具體格來電確認。

《歡迎聯(lián)系我們》

座機：021-57709403        手機：13641981733

在線QQ：858579329         聯(lián)系人：蔣芝林

請登陸公司主網址： www.dingtuosh.com

網址二：www.shdto.com

AND精密電子天平廠【252g電子天平 HR-250AZ】電子天平

 

該公司產品分類： 無動力滾筒電子秤 報警檢重秤 移動式防爆電子臺秤 滾筒稱重機 不銹鋼電子滾筒秤 流水線檢重秤 流水線滾筒電子秤 大噸位動力滾筒秤 流水線配料稱重系統(tǒng) 配料稱重系統(tǒng) 選別秤 皮帶滾筒秤 四托輥皮帶秤 全浮式三托輥皮帶稱重 三托輥皮帶秤 皮帶機稱重 輸送皮帶稱重 皮帶秤 定量檢重秤 重量檢重秤

eppendorf可調移液器　艾本德可調移液槍　eppendorf微量移液器

德國 Eppendorf 移液器

名稱 包裝 單價 （元）
1
3111型 可調移液器 0.1-2.5ul 支 1400
2
3111型 可調移液器 0.5-10ul 支 1400
3
3111型 可調移液器 2.0-20ul 支 1400
4
3111型 可調移液器 10-100ul 支 1400
5
3111型 可調移液器 20-200ul 支 1400
6
3111型 可調移液器 100-1000ul 支 1400
7
3111型 可調移液器 500-5000ul 支 1400
8
4910型 可調移液器 0.1-2.5ul      可整支高溫消毒 支 2200
9
4910型 可調移液器 0.5-10ul       可整支高溫消毒 支 2200
10
4910型 可調移液器 2.0-20ul       可整支高溫消毒 支 2200
11
4910型 可調移液器 10-100ul       可整支高溫消毒 支 2200
12
4910型 可調移液器 20-200ul       可整支高溫消毒 支 2200
13
4910型 可調移液器 100-1000ul     可整支高溫消毒 支 2200
14
4910型 可調移液器 500-2500ul     可整支高溫消毒 支 2200

100-37-8N,N-二乙基乙醇胺

 N,N-二乙基乙醇胺
中文名稱N,N-二乙基乙醇胺
英文名稱N,N-Diethylethanolamine
中文別名二乙氨基乙醇;2-二乙基氨基乙醇;二乙基乙醇胺;2-(二乙氨基)乙醇;2-羥基三乙胺;2-二乙氨基乙醇
CAS RN100-37-8
分 子 式C6H15NO
分 子 量117.1894
物化性質性狀 無色液體，微有氨臭。有吸濕性。
用　　途用作醫(yī)藥中間體、軟化劑、乳化劑、固化劑等
N,N-二乙基乙醇胺
中文名稱N,N-二乙基乙醇胺
英文名稱N,N-Diethylethanolamine
中文別名二乙氨基乙醇;2-二乙基氨基乙醇;二乙基乙醇胺;2-(二乙氨基)乙醇;2-羥基三乙胺;2-二乙氨基乙醇
CAS RN100-37-8
分 子 式C6H15NO
分 子 量117.1894
物化性質性狀 無色液體，微有氨臭。有吸濕性。
用　　途用作醫(yī)藥中間體、軟化劑、乳化劑、固化劑等
 
該公司產品分類： 中間體

ZYP600KN全自動粉末壓片機（60噸）

 ZYP-600KN全自動壓片機技術指標：壓力范圍：3-60噸壓頭最大行程：100mm 壓片保壓時間 0~999 秒 供電電源 380V/50HZ  額定功率 1.3kw 模   具：硼酸、鋼環(huán)、鋁杯和塑料環(huán) 外型尺寸 1180x720x530mm 凈重 350Kg 儀器結構：1、調節(jié)絲杠 2、壓片模具 3、立柱 4、擺臂 5、壓力表 6、操作板 7、液壓站（右側門內） 8、壓力表 9、點動上行按鈕 10、點動下行按鈕 11、壓片保壓時間調節(jié)  12、電源指示燈 13、壓樣啟動按鈕14、停止按鈕 結構特點：●內模式壓樣，壓頭行程100毫米。可用于硼酸、鋼環(huán)、鋁杯、塑環(huán)等各類壓樣方式，適用范圍廣，工作穩(wěn)定。 ●特有的緩加壓、慢退模程序更有利于保證壓樣合格率、提高壓樣樣品質量。 ●特別設計的集成液壓管道模塊不僅使組裝更加方便，而且減少了漏油機會。 ●加厚的油箱蓋板使油泵和電磁閥的動作噪音更小。●采用由可控硅集成的固態(tài)模塊替代傳統(tǒng)的接觸器避免了由于電機頻繁啟動而引起的各類故障，有效提高了該部分的使用壽命。 ●繼電器控制部分采用印刷板焊接結構，減少了控制箱的接線，提高了電器控制的可靠性。 ●控制電源全部采用低壓24V，工作更加安全可靠。 ●結構緊湊，占地面積小。

該公司產品分類： 壓片機模具 自動粉末壓片機 電動粉末壓片機 手動粉末壓片機 粉碎機

派克parker氣動、 Sinclair產品

 一級代理

聯(lián)系人：項渭良   

電話：18259177659

傳真：

H096-10H100-22H134-82H135-11H135-14H002-18H096-32H096-82H136-24H136-29H136-37H175-27H178-74H249-05HW096-002H060-15H100-48H135-51H266-39H096-48H096-49H096-52H096-56H137-24H028-23H136-43H137-44H137-51H096-95H135-93H137-20H137-36H137-55H142-06H072-80H096-101H097-09H135-111FH138-06H237-45H132-04K133-001H132-05H136-77H143-03H097-08H122-44H136-10H137-46D043-080H090-64H244-03C573-008H134-109H135-41H136-08H178-24H135-37H137-84K057-01H143-13C713-007H232-10H072-85H232-87D293-149H122-06H136-17H136-51D222-001H092-12H096-99H101-17H135-25H138-22H137-14C683-076H178-73H220-20H143-19H092-02H143-23D293-131H132-06H143-18H178-86C683-050D293-001H092-32H220-21C343-067H089-71K383-001H092-18C683-018C333-056H135-31H143-09H143-39H135-22H143-06H220-13H143-21D043-076H143-30H090-19H092-25H138-03H138-53C343-068H092-15H143-26H137-07H143-33D223-065H090-22H137-64H237-42H137-47FH143-43HW134-006H060-23D133-077C383-010C683-034C713-017H239-14H136-87H232-25D293-130H134-124K213-005H143-11K293-001C343-069C383-063H143-31C333-049H143-37H238-34C783-141D093-173H232-24HW096-004M163-005D293-002C333-063C333-010C753-085D083-018D083-023C383-019C383-022C383-064HW138-001C383-062H134-18C383-070C683-033C333-053C883-006C383-028C643-002C383-072C713-012D293-300C213-212C383-029H232-26C333-017C242-032H138-12FC553-022H092-01C383-077H089-73C713-003H092-04C383-076C383-078C963-085C242-024C383-071H220-19C242-030C333-057K383-401C333-050C713-004C383-032K293-002D293-004C293-111K383-601D043-120H090-49C213-203C853-101C683-008C573-016C643-006C242-031C553-027D043-121H145-43C383-075D123-052K383-201K383-701C963-165C353-011H143-48C242-021C783-145C333-022H145-85D043-091C333-051H143-45C343-070H143-105H062-23H143-36H092-14C333-024D083-015C242-022D043-098D293-069C353-012C783-061D293-157D293-158D293-140C293-018M423-002C353-013H143-29D153-061C583-025C973-064D093-209D153-017C583-024H092-24C383-073K032-002C783-146D093-242HW143-001C333-007C333-061C663-010H143-28C603-030H272-02D293-072C293-023K133-003H092-22C853-103C663-022C333-030D153-077D333-003H092-03C853-102D043-065D093-037C182-003D293-147D153-068D333-002H092-23C783-081C753-086H092-05C773-032C883-004H239-22C663-026C182-006F 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BSPTRC294-40022C294-4001C294-4002C284-40011C284-40071C284-40061C294-4007C294-4008C284-40081C284-40031C294-4003C294-4004C284-40041C284-40011BSPTRC514-20022C284-40021BSPTRM38402516C514-2002C284-40031BSPTRL214-1302C294-40042L214-1301C264-4014C294-40032C264-4013C244-6002C264-4006C264-4005C264-4009C264-40142C264-4010C264-4001C264-4011C264-40132C264-4002C264-4012C264-4013 BSPTRC264-4003C264-4004M39402502C264-40062C264-4001 BSPTRM39402505L214-1303M39402501M38402503K00-61016C264-40052D013-011M38402502M394-4101M384-2101M394-2101M38404503M38403501C264-40141C264-40131C274-4003M38403503C244-6001M384-3101M394-3101C274-4004C264-40061C113-054C264-40051C274-4001L214-1202K10-1C274-4002C264-4005 BSPTRC264-40052BSPTRK00-51015K10-3L214-1401M38404502M384-4101D283-059C264-40051BSPTRL214-1403K10-8C244-6003M38402504D283-082C093-114C414-20042DFBSPM39402503M384-2301M394-2301C414-20042DFVC284-6002C344-2003C284-6006M38402505L214-1402D283-104C304-2012C284-6002 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聯(lián)系人：項渭良   

電話：18259177659

傳真：

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一級代理

聯(lián)系人：項渭良   

電話：18259177659

傳真：

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一級代理

聯(lián)系人：項渭良   

電話：18259177659

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該公司產品分類： 西門子 派克

YTC012AQ-Sun Xe-3日曬色牢度測試儀

Q-Sun氙燈試驗機采用能最大吻合性地模擬自然界全陽光光譜的氙弧燈來再現(xiàn)不同環(huán)境下存在的破壞性光波，從而可以極好的對材料進行耐光性與耐候性加速試驗？捎糜谛虏牧系暮Y選、改進現(xiàn)有材料或評估材料成份變化后耐用性的變化等試驗。

該型號（XE-3）試驗機是功能齊全、全尺寸的落地型。采用 了3個獨立的氙弧燈管，并具有較大的試樣容量。同時 能對尺寸較大的三維部件等進行光照曝曬試驗。它的試 件托盤尺寸為451mm X 718mm，能同時容納39件尺寸為 2.75 X 4平方英寸的平面試件。對于進行尺寸較大的三維部件或配件的光照暴露試驗，這種大型好的托盤有特別的用處。Q-Sun Xe-3還可以對黑板溫度和空氣溫度進行控制監(jiān)測和顯示。

特點:全日光光譜

太陽眼光幅照度控制系統(tǒng)

黑板溫度和試驗箱空氣溫度控制

相對濕度控制

水噴淋功能

光幅照度的均勻性

標準:AATCC16         ISO105

Q-Sun Xe-3（大尺寸型號）

型號

燈管

太陽眼照 度控制

水噴淋

黑板溫 度控制

箱溫 控制

相對濕 度控制

冷卻器

Xe-3-B

3

√

 

√

 

 

 

Xe-3-S

3

√

√

√

 

 

 

Xe-3-H

3

√

 

√

√

√

 

Xe-3-HS

3

√

√

√

√

√

 

Xe-3-HC

3

√

 

√

√

√

√

Xe-3-HSC

3

√

√

√

√

√

√
該公司產品分類： 皮革類、鞋業(yè)類、塑料類測試儀器 YTK織物燃燒性能 YTI生態(tài)性能 YTH通用測試儀器 YTG標準耗材 YTF染色整理 YTE材料強力機 YTD尺寸穩(wěn)定性 YTC色牢度 YTB織物服裝 YTA纖維紗線 紡織類測試儀器

48T/96T大鼠阿立新A(OrexinA)ELISA試劑盒

大鼠阿立新A（OrexinA）ELISA檢測試劑盒

使用說明書

檢測原理

試劑盒采用雙抗體一步夾心法酶聯(lián)免疫吸附試驗（ELISA）。往預先包被大鼠阿立新A（OrexinA）捕獲抗體的包被微孔中，依次加入標本、標準品、HRP標記的檢測抗體，經過溫育并洗滌。用底物TMB顯色，TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的大鼠阿立新A（OrexinA）呈正相關。用酶標儀在450nm 波長下測定吸光度（OD 值），計算樣品濃度。

樣品收集、處理及保存方法

1. 血清：使用不含熱原和內毒素的試管，操作過程中避免任何細胞刺激，收集血液后，3000轉離心10分鐘將血清和紅細胞迅速小心地分離。

2. 血漿：EDTA、檸檬酸鹽或肝素抗凝。3000轉離心30分鐘取上清。

3. 細胞上清液：3000轉離心10分鐘去除顆粒和聚合物。

4. 組織勻漿：將組織加入適量生理鹽水搗碎。3000轉離心10分鐘取上清。

5. 保存：如果樣本收集后不及時檢測，請按一次用量分裝，凍存于-20℃，避免反復凍融，在室溫下解凍并確保樣品均勻地充分解凍。

自備物品

1.     酶標儀（450nm）

2.     高精度加樣器及槍頭：0.5-10uL、2-20uL、20-200uL、200-1000uL

3.     37℃恒溫箱

操作注意事項

1. 試劑盒保存在2-8℃，使用前室溫平衡20分鐘。從冰箱取出的濃縮洗滌液會有結晶，這屬于正，F(xiàn)象，水浴加熱使結晶溶解后再使用。

2. 實驗中不用的板條應立即放回自封袋中，密封（低溫干燥）保存。

3. 標準品稀釋液即可視為陰性對照或者空白；預處理后的樣本無需稀釋，直接取10μL加樣即可。

4. 嚴格按照說明書中標明的時間、加液量及順序進行溫育操作。

5. 所有液體組分使用前充分搖勻。

試劑盒組成

名稱

 96孔配置

 48孔配置

 備注

微孔酶標板

 12孔×8條

 12孔×4條

 無

標準品（800 pg/mL）

 0.6mL

0.6mL

按說明書進行稀釋

標準品稀釋液

 6mL

 3mL

 無

樣本稀釋液

 6mL

 3mL

 無

檢測抗體-HRP

 10mL

 5mL

 無

20×洗滌緩沖液

 25mL

 15mL

 按說明書進行稀釋

底物A

 6mL

 3mL

 無

底物B

 6mL

 3mL

 無

終止液

 6mL

 3mL

 無

封板膜

 2張

 2張

 無

說明書

 1份

 1份

 無

自封袋

 1個

 1個

 無

注：標準品用標準品稀釋液依次稀釋為：800、400、200、100、50、25 pg/mL

試劑的準備

 20×洗滌緩沖液的稀釋：蒸餾水按1：20稀釋，即1份的20×洗滌緩沖液加19份的蒸餾水。

洗板方法

1. 手工洗板：甩盡孔內液體，每孔加滿洗滌液，靜置1min后甩盡孔內液體，在吸水紙上拍干，如此洗板5次。

2. 自動洗板機：每孔注入洗液350μL，浸泡1min，洗板5次。

操作步驟

1. 從室溫平衡20min后的鋁箔袋中取出所需板條，剩余板條用自封袋密封放回4℃。

2. 設置標準品孔和樣本孔，標準品孔各加不同濃度的標準品50μL；

3. 待測樣本孔先加待測樣本10μL，再加樣本稀釋液40μL；

4. 隨后標準品孔和樣本孔中每孔加入辣根過氧化物酶（HRP）標記的檢測抗體100μL，用封板膜封住反應孔，37℃水浴鍋或恒溫箱溫育60min。

5. 棄去液體，吸水紙上拍干，每孔加滿洗滌液，靜置1min，甩去洗滌液，吸水紙上拍干，如此重復洗板5次（也可用洗板機洗板）。

6. 每孔加入底物A、B各50μL，37℃避光孵育15min。

7. 每孔加入終止液50μL，15min內，在450nm波長處測定各孔的OD值。

結果判斷

 繪制標準曲線：在Excel工作表中，以標準品濃度作橫坐標，對應OD值作縱坐標，繪制出標準品線性回歸曲線，按曲線方程計算各樣本濃度值。

試劑盒性能

1.  性：標準品線性回歸與預期濃度相關系數(shù)R值，大于等于0.9900。

2.  靈敏度：檢測濃度小于10 pg/mL。

3.  特異性：不與其它可溶性結構類似物交叉反應。

4.  重復性：板內、板間變異系數(shù)均小于15%。

5.  貯藏：2-8℃，避光防潮保存。

6.  期：6個月

免責聲明

1.   試劑盒僅供研究使用，不得用于臨床實驗或人體實驗，否則所產生的一切后果，由實驗者承擔，本公司概不負責。

2.   嚴格按照說明書操作，實驗者違反說明書操作，后果由實驗者承擔。

 

 

FOR RESEARCH USE ONLY.

NOT FOR USE IN DIAGNOSTIC PROCEDURES.

 

Rat OrexinA （OrexinA） ELISA Kit instruction

 

Intended use

This OrexinA ELISA kit is intended Laboratory for Research use only ａnd is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow ａnd the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of OrexinA in the sample, this OrexinA ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples ａnd allow the operator to produce a standard curve of Optical Density versus OrexinA concentration. The concentration of OrexinA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Sample collection ａnd storages

Serum - Use a serum separator tube ａnd allow samples to clot for 30 minutes before centrifugation for 10 minutes at approximately 3000×g. Remove serum ａnd assay immediately or aliquot ａnd store samples at -20℃ or -80℃.Avoid repeated freeze-thaw cycles

Plasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30 minutes at 3000×g at 2-8℃ within 30 minutes of collection. Store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.

Cell culture supernates ａnd other biological fluids - Remove particulates by centrifugation ａnd assay immediately or aliquot ａnd store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.

Note: The samples shoule be centrifugated dequately ａnd no hemolysis or granule was allowed.

Materials required but not supplied

1. Standard microplate reader（450nm）

2. Precision pipettes ａnd Disposable pipette tips.

3. 37 ℃ incubator

Precautions

1.  Do not substitute reagents from one kit to another. Standard, conjugate and microplates are matched for optimal performance. Use only the reagents supplied by manufacturer.

2. Do not remove microplate from the storage bag until needed. Unused strips should be stored at 2-8°C in their pouch with the desiccant provided.

3.  Mix all reagents before using.

Remove all kit reagents from refrigerator ａnd allow them to reach room temperature （ 20-25°C）

Materials supplied

Name

 96 determinations

 48 determinations
Microelisa stripplate

 12*8strips

 12*4strips
Standard（800 pg/mL）

 0.6ml

 0.6ml
Standard diluent

 6.0ml

 3.0ml
Sample diluent

 6.0ml

 3.0ml
HRP-Conjugate reagent

 10.0ml

 5.0ml
20X Wash solution

 25ml

 15ml
Chromogen Solution A

 6.0ml

 3.0ml
Chromogen Solution B

 6.0ml

 3.0ml
Stop Solution

 6.0ml

 3.0ml
Closure plate membrane

 2

 2
User manual

 1

 1
Sealed bags

 1

 1

Note: Standard diluent with Standard diluent concentration was followed by:

800、400、200、100、50、25 pg/mL

Reagent preparation

20×wash solution:Dilute with Distilled or deionized water 1:20.

 

Assay procedure

1.  Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.

2.  Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.

3. Add Sample: Add testing sample 10μl Then add sample diluent 40μl to testing sample well; Blank well doesn’t add anyting.

4. Add 100μl of HRP-conjugate reagent to each well, cover with an adhesive strip and incubate for 60 minutes at 37°C.

5.  Aspirate each well ａnd wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution （400μl） using a squirt bottle, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting. Invert the plate ａnd blot it against clean paper towels.

6.  Add chromogen solution A 50μl ａnd chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.

7.  Add 50μl Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not

 

appear uniform, gently tap the plate to ensure thorough mixing.

8.  Read the Optical Density （O.D.） at 450 nm using a microtiter plate reader within 15 minutes.

Calculation of results

1.         This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. （450 nm） obtained for each of the six standard concentrations on the vertical （Y） axis versus the corresponding concentration on the horizontal （X） axis.

2.         First, calculate the mean O.D. value for each standard ａnd sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software.

3.         To determine the amount in each sample, first locate the O.D. value on the Y-axis ａnd extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis ａnd read the corresponding concentration.

4.         Any variation in operator, pipetting ａnd washing technique, incubation time or temperature, ａnd kit age can cause variation in result. Each user should obtain their own standard curve.

5.         The sensitivity by this assay is 10 pg/mL.

6.         Standard curve

 

Storage： 2-8℃.

validity： six months.

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

郵箱：yupingbio@126.com公司展臺: http://www.yupingbio.com
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